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What have we demonstrated in our research?

“Extraordinary Claims require Extraordinary Evidence” ~ Carl Sagan

1. Effects on proteins expression in micro- organisms

a. A large number of valuable native proteins /enzymes/coenzymes of different kinds were modulated / expressed without genetic engineering processes after exposing microorganisms (Saccharomyces Cerevisia yeasts and E coli) for a few minutes to the Plasma Modified Field generated by Orteron’s bio-technology. These proteins are unique to the experimental samples and were not modulated in the non-treated samples.

b. About half of the yeast-based native proteins modulated by our bio-technology are available in the protein market mostly as recombinant protein; About a quarter of the modulated proteins exists in the market only as partial (incomplete structure) recombinant proteins; and about one quarter of the proteins expressed by our technology are very difficult to produce as evidenced by its seldom existence in the protein market, despite their importance and potential value.

Examples of several of the yeast-based native proteins modulated by Orteron’s technology which are hardly found in the protein market  

Q07793  Transposon Ty1-DR4 Gag-Pol Polyprotein (gene TY1B-DR4);

Q02793 Antiviral Protein SKI8 ((gene SKI8);

Q03834  DNA mismatch repair protein (gene ULS1);

Q08220  Glutathione synthase ((GSS), Gene GSH2);

Q05567  Sphingosine-1-phosphate lyase  (Gene DPL1);

Examples of several of the protein modulated by Orteron’s biotechnology which are available in the market as partial recombinant proteins

Q12018  Cell division control protein 53 (gene CDC53);

P35207  Antiviral helicase SK12 (gene SKI2);

P38604  Lanosterol Synthase (gene ERG7);

P15424  ATP dependent RNA helicase, mitochondrial;

P06839  DNA repair helicase RAD3 (Gene RAD3)

Examples of several of the proteins modulated by Orteron’s biotechnology which are available in the market mostly as recombinant proteins

P04803 Tryptophan–tRNA ligase, mitochondrial (Gene MSW1);

P36014 Glutathione peroxidase 1 (Gene GPX1);

P48524 Ubiquitin ligase-binding protein BUL1 (Protein BUL1);

Q12246 Sphingoid long chain base kinase 4  (GeneLCB4);

P53220 Mitochondrial import inner membrane translocase subunit TIM21

c. The yeasts cells’ exposed to the Plasma Modified Field of Orteron’s biotechnology generate the following 9 de-novo intarcellular pathways that are not normally found in the metabolic processes of non-treated yeasts cells: Angiogenesis ; VEGF signaling ; Cell Cycle ; Cytoskeletal regulation by Rho GTpase;  Methionine Biosynthesis;  PDGF Signaling pathway; Purine Metabolism; Tryptophan Biosynthesis ; mRNA Splicing.

Controlled based Intracellular pathways   Orteron’s Treated-based pathways

d. Modifications triggered by the exposure to the Modified Plasma Field for a few minutes result in changes of spot patterns of the same identified protein. Modifications mediated by Orteron’s Modified Plasma field treatment often result in permanent and sometimes irreversible structural changes. The Gel represents treatment versus control beyong time  of measurement. The analysis is based on both: MALDI results and CS identification and 2D DIGE and ratios

2. Effects on Recombinant Proteins

  • When Orteron’s Plasma Modified Field has been applied on recombinant proteins and enzymes, using two different time duration of operation, no changes has occurred to the recombinant proteins structure and to the enzymatic activity
  • Forthcoming study aims to demonstrate that when intervening in the engineering process of a protein (in yeast and E coli) the application of Orteron’s Modified Plasma Field will significantly increase the amount of the proteins produced

3. Producing Biofuel

When Orteron’s bio-technology is applied on yeast and bacteria it provides fuel alternatives and basic materials for different industries having multiple practical applications (examples: pharmaceutical applications; coating, printing, ink, plastic and food industry and as a direct solvent).

The bio-technology provides advantageous and beneficial next generation bio-fuel and other molecules/compounds, based on revolutionary metabolic and bio-chemical efficient processes thereby producing diverse compounds such as:

1-Propanol                          CH3(CH2)2OH

2-Propanol (isopropanol)   (CH3)2CHOH

2-3 Butanediol   (2-3BD)    (CH3)2(CHOH)2

Butanedione                         (CH3CO)2

Acetoine                               (C4H8O2)

4. Deactivation of Bacteria

When applied on different kinds of bacteria, Orteron’s bio-technology is capable of deactivating bacteria within few minutes (examples: Listeria and E coli). The rate and the amount of bacteria deactivation depends on the time length of the bio-technology operation.

5. Stability and Change in Water

When Orteron’s modified plasma field is applied on both mineral and distilled water, the water PH did not change for every number of time length used; No nitrate has been found in the water for all the time duration used; Nitrite has been measured after about 3.5 minutes of use and stayed relatively constant around between 0.15 and 0.20 m per liter; Hydrogen peroxide has not been produced during the first 10 minutes of the bio-technology operation and only after about ten  minutes hydrogen peroxide has been revealed and grown in the water