“Extraordinary Claims require Extraordinary Evidence” ~ Carl Sagan
1. Effects on messenchymal stem cells / Stromal cells
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Epigenetic regulation relative to control
Upreguilated Genes based on two different treatments on stem cells study 1
Downregulated genes of two difefrent treatments on stem cells study 1
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Effects on relationships between epigenetic regulation and diseases and functions of stem cells, relative to control
Effects on relationships between epigenetic regulation and diseases when acting on stem cells
2. Effects on proteins expression in micro-organisms
a. A large number of valuable native proteins /enzymes/coenzymes of different kinds were modulated / expressed without genetic engineering processes after exposing microorganisms (Saccharomyces Cerevisia yeasts and E coli) for a few minutes to the Plasma Modified Field generated by Orteron’s bio-technology, Teteron’s Protein Reset Technology (TPRT). These proteins are unique to the experimental samples and were not modulated in the non-treated samples.
b. About half of the yeast-based native proteins modulated by TPRT are available in the protein market mostly as recombinant protein; About a quarter of the modulated proteins exists in the market only as partial (incomplete structure) recombinant proteins; and about one quarter of the proteins expressed by our technology are very difficult to produce as evidenced by its seldom existence in the protein market, despite their importance and potential value.
Examples of several of the yeast-based native proteins modulated by Orteron’s technology which are hardly found in the protein market
Q07793 Transposon Ty1-DR4 Gag-Pol Polyprotein (gene TY1B-DR4);
Q02793 Antiviral Protein SKI8 ((gene SKI8);
Q03834 DNA mismatch repair protein (gene ULS1);
Q08220 Glutathione synthase ((GSS), Gene GSH2);
Q05567 Sphingosine-1-phosphate lyase (Gene DPL1);
Examples of several of the protein modulated by Orteron’s biotechnology OPRT which are available in the market as partial recombinant proteins:
Q12018 Cell division control protein 53 (gene CDC53);
P35207 Antiviral helicase SK12 (gene SKI2);
P38604 Lanosterol Synthase (gene ERG7);
P15424 ATP dependent RNA helicase, mitochondrial;
P06839 DNA repair helicase RAD3 (Gene RAD3)
Examples of several of the proteins modulated by Orteron’s biotechnology OPRT which are available in the market mostly as recombinant proteins
P04803 Tryptophan–tRNA ligase, mitochondrial (Gene MSW1);
P36014 Glutathione peroxidase 1 (Gene GPX1);
P48524 Ubiquitin ligase-binding protein BUL1 (Protein BUL1);
Q12246 Sphingoid long chain base kinase 4 (GeneLCB4);
P53220 Mitochondrial import inner membrane translocase subunit TIM21
c. The yeasts cells’ exposed to the Plasma Modified Field of Orteron’s biotechnology (TPRT) generate the following 9 de-novo intarcellular pathways that are not normally found in the metabolic processes of non-treated yeasts cells: Angiogenesis ; VEGF signaling ; Cell Cycle ; Cytoskeletal regulation by Rho GTpase; Methionine Biosynthesis; PDGF Signaling pathway; Purine Metabolism; Tryptophan Biosynthesis ; mRNA Splicing.
Controlled based Intracellular pathways TPRT Treated-based pathways
d. Modifications triggered by the exposure to the Modified Plasma Field for a few minutes result in changes of spot patterns of the same identified protein. Modifications mediated by Orteron’s Modified Plasma field treatment often result in permanent and sometimes irreversible structural changes. The Gel represents treatment versus control beyong time of measurement. The analysis is based on both: MALDI results and CS identification and 2D DIGE and ratios
3. Effects on Recombinant Proteins
When Orteron’s Non-Thermal Plasma Modified Field has been applied on recombinant proteins and enzymes, using two different time duration of operation, no changes has occurred to the recombinant proteins structure and to the enzymatic activity. Also when interferred with engineering processes to modulate the number of recombinant proteins produced, no changes has occured. These results confirm our hypothesis that Tetron Protein Rest Technology affects in-vivo biological-physiological and chemical processes ocurring naturally inside the organism (microorganism, plant, human being). It does not affect any processes which are externally intervened- altered or modulated.
In other words the strength of the Tetron Protein Reset Technology (TPRT) is its power to alter or modulate or regulate or heal naturally occurring biological, chemical and physiological life processes that are vital for a targeted organism (being microorganism, a plant, animals or a human being) to live and to shape its capabilities to interact with the environment.
4. Producing Biofuel
When Orteron’s bio-technology,TPRT, is applied on yeast and bacteria it provides fuel alternatives and basic materials for different industries having multiple practical applications (examples: pharmaceutical applications; coating, printing, ink, plastic and food industry and as a direct solvent).
TPRT provides advantageous and beneficial next generation bio-fuel and other molecules/compounds, based on revolutionary metabolic and bio-chemical efficient processes thereby producing diverse compounds such as:
1-Propanol CH3(CH2)2OH
2-Propanol (isopropanol) (CH3)2CHOH
2-3 Butanediol (2-3BD) (CH3)2(CHOH)2
Butanedione (CH3CO)2
Acetoine (C4H8O2)
5. Deactivation of Bacteria
When applied on different kinds of bacteria, Orteron’s bio-technology, TPRT, is capable of deactivating bacteria within few minutes (examples: Listeria and E coli). The rate and the amount of bacteria deactivation depends on the time length of the bio-technology operation.
5. Stability and Change in Water
When Orteron’s modified plasma field, generated by TPRT, is applied on both mineral and distilled water, the water PH did not change for every number of time length used; No nitrate has been found in the water for all the time duration used; Nitrite has been measured after about 3.5 minutes of use and stayed relatively constant around between 0.15 and 0.20 m per liter; Hydrogen peroxide has not been produced during the first 10 minutes of the bio-technology operation and only after about ten minutes hydrogen peroxide has been revealed and grown in the water